A gene-derived SNP-based high resolution linkage map of carrot including the location of QTL conditioning root and leaf anthocyanin pigmentation

BMC Genomics

Philipp W Simon³  Shelby Ellison¹  Joshua Parsons¹  Douglas Senalik³  Mehtap Yildiz⁴  Massimo Iorizzo¹  Pablo F Cavagnaro² 

[1]Department of Horticulture, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53706, USA

[2]CONICET, Facultad de Ciencias Agrarias – Universidad Nacional de Cuyo, and INTA E.E.A. La Consulta, Ex Ruta 40. km 96, La Consulta CC 8, Mendoza 5567, Argentina

[3]USDA-Agricultural Research Service, Vegetable Crops Unit, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53706, USA

[4]Department of Agricultural Biotechnology, Faculty of Agriculture, Yuzuncu Yil University, 65080 Van, Turkey

关键词: Single nucleotide polymorphism;    Linkage map;    QTL mapping;    Anthocyanins;    Carrot;   

Others  :  1127224
DOI  :  10.1186/1471-2164-15-1118

 received in 2014-09-11, accepted in 2014-12-11,  发布年份 2014

【 摘 要 】

Background

Purple carrots accumulate large quantities of anthocyanins in their roots and leaves. These flavonoid pigments possess antioxidant activity and are implicated in providing health benefits. Informative, saturated linkage maps associated with well characterized populations segregating for anthocyanin pigmentation have not been developed. To investigate the genetic architecture conditioning anthocyanin pigmentation we scored root color visually, quantified root anthocyanin pigments by high performance liquid chromatography in segregating F₂, F₃ and F₄ generations of a mapping population, mapped quantitative trait loci (QTL) onto a dense gene-derived single nucleotide polymorphism (SNP)-based linkage map, and performed comparative trait mapping with two unrelated populations.

Results

Root pigmentation, scored visually as presence or absence of purple coloration, segregated in a pattern consistent with a two gene model in an F₂, and progeny testing of F₃-F₄ families confirmed the proposed genetic model. Purple petiole pigmentation was conditioned by a single dominant gene that co-segregates with one of the genes conditioning root pigmentation. Root total pigment estimate (RTPE) was scored as the percentage of the root with purple color.

All five anthocyanin glycosides previously reported in carrot, as well as RTPE, varied quantitatively in the F₂ population. For the purpose of QTL analysis, a high resolution gene-derived SNP-based linkage map of carrot was constructed with 894 markers covering 635.1 cM with a 1.3 cM map resolution. A total of 15 significant QTL for all anthocyanin pigments and for RTPE mapped to six chromosomes. Eight QTL with the largest phenotypic effects mapped to two regions of chromosome 3 with co-localized QTL for several anthocyanin glycosides and for RTPE. A single dominant gene conditioning anthocyanin acylation was identified and mapped.

Comparative mapping with two other carrot populations segregating for purple color indicated that carrot anthocyanin pigmentation is controlled by at least three genes, in contrast to monogenic control reported previously.

Conclusions

This study generated the first high resolution gene-derived SNP-based linkage map in the Apiaceae. Two regions of chromosome 3 with co-localized QTL for all anthocyanin pigments and for RTPE, largely condition anthocyanin accumulation in carrot roots and leaves. Loci controlling root and petiole anthocyanin pigmentation differ across diverse carrot genetic backgrounds.

【 授权许可】

   
2014 Cavagnaro et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

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Figure 4.

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