Real-time in vivo monitoring of circadian E-box enhancer activity: A robust and sensitive zebrafish reporter line for developmental, chemical and neural biology of the circadian clock

Developmental biology

MeltemWeger¹ 

关键词: Circadian clock;    Zebrafish;    E-box;    Luciferase reporter;    Adult brain;    Neurogenesis;   

DOI  :  10.1016/j.ydbio.2013.04.035

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

The circadian clock co-ordinates physiology and behavior with the day/night cycle. It consists of a transcriptional–translational feedback loop that generates self-sustained oscillations in transcriptional activity with a roughly 24 h period via E-box enhancer elements. Numerous in vivo aspects of core clock feedback loop function are still incompletely understood, including its maturation during development, tissue-specific activity and perturbation in disease states. Zebrafish are promising models for biomedical research due to their high regenerative capacity and suitability for in vivo drug screens, and transgenic zebrafish lines are valuable tools to study transcriptional activity in vivo during development.

【 授权许可】

CC BY