Detection and molecular characterization of equine infectious anemia virus in Mongolian horses

The Journal of Veterinary Medical Science

Yoshihiro SAKODA¹  Satoru KONNAI²  Takashi UMEMURA²  Tungalag CHULTEMDORJ²  Nyamsuren OCHIRKHUU³  Tumenjargal SHARAV³  Kazuhiko OHASHI³  Shiro MURATA³  Erdene Ochir TS³  Hirohisa MEKATA⁴ 

[1]Center for Animal Disease Control, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki 889-2192, Japan

[2]Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, Japan

[3]Department of Infectious Diseases and Public Health, School of Veterinary Medicine, Mongolian University of Life Science, Khan-Uul district, Zaisan 17042, Ulaanbaatar, Mongolia

[4]Organization for Promotion of Tenure Track, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki 889-2192, Japan

关键词: equine infectious anemia virus;    horse;    LTR-gene;    molecular epidemiology;    Mongolia;   

DOI  :  10.1292/jvms.17-0202

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

The genetic characterization and actual prevalence of EIAV in Mongolian horse in the disease endemic region is currently unknown. Here, 11 of 776 horse serum samples from four Mongolian provinces tested positive on agar gel immunodiffusion test. Genomic DNA extracted from all seropositive samples was subjected to nested PCR assay. Among these, three samples tested positive with nested PCR assay and were identified by sequencing analysis based on long termination repeat and tat gene of the virus. Two of the three sequences were identical, with 94.0% identity with the third. These two independent Mongolian EIAV sequences were retained functional motifs, with no dramatic changes but some variability in the U5 region; they were clustered with genotypes from European countries but not with those from China, U.S.A., or Japan.

【 授权许可】

Unknown