期刊论文详细信息
The Journal of Veterinary Medical Science
Detection and molecular characterization of equine infectious anemia virus in Mongolian horses
Yoshihiro SAKODA1  Satoru KONNAI2  Takashi UMEMURA2  Tungalag CHULTEMDORJ2  Nyamsuren OCHIRKHUU3  Tumenjargal SHARAV3  Kazuhiko OHASHI3  Shiro MURATA3  Erdene Ochir TS3  Hirohisa MEKATA4 
[1] Center for Animal Disease Control, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki 889-2192, Japan;Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, Japan;Department of Infectious Diseases and Public Health, School of Veterinary Medicine, Mongolian University of Life Science, Khan-Uul district, Zaisan 17042, Ulaanbaatar, Mongolia;Organization for Promotion of Tenure Track, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki 889-2192, Japan
关键词: equine infectious anemia virus;    horse;    LTR-gene;    molecular epidemiology;    Mongolia;   
DOI  :  10.1292/jvms.17-0202
学科分类:兽医学
来源: Japanese Society of Veterinary Science
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【 摘 要 】

The genetic characterization and actual prevalence of EIAV in Mongolian horse in the disease endemic region is currently unknown. Here, 11 of 776 horse serum samples from four Mongolian provinces tested positive on agar gel immunodiffusion test. Genomic DNA extracted from all seropositive samples was subjected to nested PCR assay. Among these, three samples tested positive with nested PCR assay and were identified by sequencing analysis based on long termination repeat and tat gene of the virus. Two of the three sequences were identical, with 94.0% identity with the third. These two independent Mongolian EIAV sequences were retained functional motifs, with no dramatic changes but some variability in the U5 region; they were clustered with genotypes from European countries but not with those from China, U.S.A., or Japan.

【 授权许可】

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