Cinco décadas de la biología molecular y la citogenética aplicadas a la hematología cubana

Revista Cubana de Hematología, Inmunología y Hemoterapia

Garrote Santana, Heidys¹  Amor Vigil, Ana María¹  Díaz Alonso, Carmen¹  Rivero Jiménez, René A¹  Fernández Martínez, Lesbia¹  Ruiz Moleón, Vera¹  Hernández Aguilar, Norbelys¹  Lavaut-Sánchez, Kalia¹ 

[1] Instituto de Hematología e Inmunología, La Habana, Cuba

关键词: molecular biology;    molecular techniques;    diagnosis.;   

DOI  :  

学科分类：生理学与病理学

来源: SciELO

【 摘 要 】

Molecular biology (MB) is a science that has revolutionized scientific development in recent years. It has also increasing progressively at the Institute of Hematology and Immunology (IHI) as adapting to technological advances and international scientific context. Its history dates back to 1966, with the IHI creation and subsequently the MB laboratory. Since then they have been many achievements in the field of diagnosis and research in Hematology. In 2012, the MB department with the cytogenetic laboratory was part of the Center for Advanced Technologies; an area with modern technology that has allowed change old studies by updated molecular techniques, ensuring greater speed and reliability of results. Techniques such as extraction and quantification of nucleic acids (NA), capillary electrophoresis and the FISH (Fluorescence in Situ Hybridization) were introduced, and modern thermocyclers for polymerase chain reaction (PCR), materials and reagents were acquired too. In these 50 years, 5 460 patients have been benefited to date. We study about 10 molecular markers, 12 FISH study, in addition to conventional karyotyping and chimerism studies in the MB lab at this moment. It has gone an annual average of 317 patients in the last 5 years. We have highly qualified professionals, which has made possible to lead and collaborate on national and international research, publishing numerous scientific articles, obtain relevant prizes of science and technology forum and directly contribute to the residents' formation in Hematology. Our future perspectives include the new technologies incorporation such as real-time PCR and sequencing, to complete a similar diagnostic level to any prestigious international center so we can provide quality service to our patients.

【 授权许可】

CC BY-NC-SA