Pre-Treatment with Metformin in Comparison with Post-Treatment Reduces Cerebral Ischemia Reperfusion Induced Injuries in Rats

Bulletin of Emergency and Trauma

Sara Shojaei Zarghani¹  Hamid Soraya²  Majid Mohajer Milani²  Mojtaba Karimipour³ 

[1] Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran;Department of Pharmacology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran;Neurophysiology Research Center, Department of Anatomy, Urmia University of Medical Sciences, Urmia, Iran

关键词: Global cerebral ischemia;    Metformin;    Oxidative stress;    Inflammation;   

DOI  :  

学科分类：基础医学

来源: Shiraz University of Medical Sciences

【 摘 要 】

Objective:To explore the effects of preversuspost ischemic treatment with metformin after global cerebral ischemia in rats. Methods : Male Wister rats underwent forebrain ischemia by bilateral common carotid artery occlusion for 17 min. Metformin (200 mg/kg) or vehicle was given orally by gavage for 7-14 days. Rats were divided into: control, metformin pre-treatment, metformin post-treatment and metformin pre and post continuous treatment groups. Cerebral infarct size, histopathology, myeloperoxidase and serum malondialdehyde were measured 7 days after ischemia. Results:Histopathological analysis showed that metformin pre-treatment significantly decreased leukocyte infiltration, myeloperoxidase activity and also malondialdehyde level. Metformin pre-treatment and metformin post-treatment reduced infarct size compared with the control group, but it was not significant in the pre and post continuous treatment group. Conclusion:Our findings suggest that pre-treatment with metformin in comparison with post-treatment in experimental stroke can reduce the extent of brain damage and is more neuroprotective at least in part by inhibiting oxidative stress and inflammation.

【 授权许可】

CC BY