Inflammation and NFκB activation is decreased by hypothermia following global cerebral ischemia

NEUROBIOLOGY OF DISEASE

卷:33

Article

Webster, Carla M.^1,2,3  Kelly, Stephen⁴  Koike, Maya A.^1,2,3  Chock, Valerie Y.³  Giffard, Rona G.³  Yenari, Midori A.^1,2 

[1] Univ Calif San Francisco, Dept Neurol, San Francisco, CA 94121 USA

[2] San Francisco VA Med Ctr, San Francisco, CA 94121 USA

[3] Stanford Univ, Sch Med, Dept Anesthesia, Stanford, CA 94305 USA

[4] Univ Bristol, Bristol BS1 3NY, Avon, England

关键词: Global cerebral ischemia;    Hypothermia;    Inflammation;    Nuclear factor-kappa B;    Microglia;   

DOI  :  10.1016/j.nbd.2008.11.001

来源: Elsevier

【 摘 要 】

We previously showed that hypothermia attenuates inflammation in focal cerebral ischemia (FCI) by suppressing activating kinases of nuclear factor-kappa B (NF kappa B). Here we characterize the inflammatory response in global cerebral ischemia (GCI), and the influence of mild hypothermia. Rodents were subjected to GCI by bilateral carotid artery occlusion. The inflammatory response was accompanied by microglial activation, but not neutrophil infiltration, or blood brain barrier disruption. Mild hypothermia reduced CA1 damage, decreased microglial activation and decreased nuclear NF kappa B translocation and activation. Similar anti-inflammatory effects of hypothermia were observed in a model of pure brain inflammation that does not cause brain cell death. Primary microglial cultures subjected to oxygen glucose deprivation (OGD) or stimulated with LPS under hypothermic conditions also experienced less activation and less NF kappa B translocation. However, NF kappa B regulatory proteins were not affected by hypothermia. The inflammatory response following GCI and hypothermia's anti-inflammatory mechanism is different from that observed in FCI. Published by Elsevier Inc.

【 授权许可】

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