Inhibition of NF-κB activation improves insulin resistance of L6 cells

Endocrine journal

Zhixing Ren¹  Fanzhu Meng²  Shuping Ren²  Jian Zhu²  Yuan Wang²  Fengyang Xie²  Baolian Hou² 

[1] Center for Disease Prevention and Control of Changchun, Jilin Province, China;Department of Occupational Health and Environmental Health, School of Public Health, Jilin University, Changchun 130021, Jilin Province, China

关键词: NF-κ    B activation;    Oxidative stress;    Insulin resistance;    Glucose uptake;    Glut4;   

DOI  :  10.1507/endocrj.EJ17-0012

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

To explore the role of NF-κB activation in the development of insulin resistance and investigate whether or not that the inhibition of NF-κB activation by PDTC will improve the insulin resistance of L6 cells exposed to H2O2.L6 cells were treated with H2O2, PDTC or both H2O2 and PDTC for 4 hours.The uptake of glucose with stimulation of insulin, the expression of P38-MAPK, p- P38-MAPK, NF-κBp65, p- NF-κBp65, IRS-1, IRS-2, p-IRS-2, PI3K, IκBα, p- IκBα, caspase-8 and GLUT4, the production of ROS, TNF-α, IL-6 and IL-1β as well as the apoptosis rate of L6 cells were determined and compared in L6 treated with H2O2 alone or both H2O2 and PDTC.Compared with the L6 cells treated with H2O2 alone, the L6 cells treated with both H2O2 and PDTC showed (1) significantly lower production of ROS, TNF-α, IL-6 and IL-1β; (2) significantly decreased expression of P38-MAPK, p- P38-MAPK and NF-κBp65, p- NF-κBp65, p- IκBα and caspase-8; (3) significantly lower rate of apoptosis; (4) significantly higher expression of IRS-2, p-IRS-2 (Tyr 612), PI3K and GLUT4; (5) significantly higher uptake of glucose with stimulation of insulin; (6) significantly increased expression of Bcl2 and decreased ratio of Bax to Bcl2.Based on the findings of the present study, inhibition of NF-κB activation by PDTC would improve the insulin resistance of L6 cells exposed to H2O2.

【 授权许可】

Unknown