Determination of vortioxetine in human serum and saliva samples by HPLC–DAD and HPLC–MS

Acta Chromatographicae

M. Waksmundzka-Hajnos¹  A. Petruczynik¹  K. Wróblewski¹  B. Buszewski²  M. Szultka-Młyńska³  H. Karakuła-Juchnowicz⁴ 

[1] 1Department of Inorganic Chemistry Medical University of Lublin, Chodźki 4a 20-093 Lublin, Poland;2Department of Environmental Chemistry and Bioanalytics, Nicolaus Copernicus University, Faculty of Chemistry Gagarina 7, PL-87-100 Toruń, Poland;3Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Wileńska 4, Toruń, Poland;4Department of Clinical Neuropsychiatry, Medical University of Lublin, Głuska 2, 20-439 Lublin, Poland

关键词: vortioxetine;    HPLC-DAD;    HPLC-MS;    human serum;    human saliva;   

DOI  :  10.1556/1326.2017.29.3.02

学科分类：化学（综合）

来源: Akademiai Kiado Rt.

【 摘 要 】

Vortioxetine is a new drug against major depressive disorder with high affinity for a range of different serotonergic targets in the central nervous system. Therapeutic drug monitoring is an important tool for the clinical management of patients receiving a pharmacotherapy, particularly in psychiatry. For this reason, determination of drug concentration in biological fluids is important for a rational dosage of drugs. Rapid and reliable analytical assays are also required to detect and identify drugs of toxicological importance. For analysis of vortioxetine by high-performance liquid chromatography (HPLC), no procedures for its determination in saliva have been reported and there are only a few ones for its determination in serum. A sensitive and selective highperformance liquid chromatography with diode array detector (HPLC-DAD) or mass spectrometer (HPLC-MS) method was developed for the fast quantification of vortioxetine in human saliva and serum. The determination was performed on a Synergi Polar RP column in isocratic mode under the optimal mobile phase containing 70% methanol, 20% acetate buffer at pH 3.5, 10% double distilled water, and 0.025 M L−1 diethylamine.

【 授权许可】

Unknown