Effect of hexane extract of Dillenia indica seed on the activity of secreted aspartyl proteinase of Candida albicans and its kinetic studies

Dynamic Chiropractic

Patrick Govender¹  O. V. S. Reddy²  P.V. G. K. Sarma³  K. Himakar Reddy⁴ 

[1] Biochemistry, University of KwaZulu-Natal, Durban-4000, South Africa;Department of Biochemistry, Sri Venkateswara University, Tirupati-517 502, India.;Department of Biotechnology, Sri Venkateswara Institute of Medical Sciences, Tirupati, India;Department of 

关键词: Dillenia indica;    Candida albicans;    secreted aspartyl proteinase;    Michaelis-menten;    Hanes-Woolf.;   

DOI  :  10.5897/JMPR10.991

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Secreted aspartyl proteinase (Sap), an extracellular enzyme ofCandida albicans,is implicated as a pathogenic factor that plays a vital role in invading and colonizing the host tissue under predisposing conditions. The present study was taken up to study the inhibitory effect of hexane extract ofDillenia indicaseed on Sap and its enzyme kinetics. Sap activity was determined spectrophotometrically at 280 nm using BSA. The enzyme kinetics of Sap showed velocity (Vmax) of 0.058 µg/mg/min and Km 0.174 µM. HoweverC. albicansgrown in different concentrations of hexane extract ofD. indicatotally inactivated the enzyme, which was further confirmed by SDS-PAGE.

【 授权许可】

Unknown