Quercetin induces apoptosis and necroptosis in MCF-7 breast cancer cells

Bratislava Medical Journal

L. Khorsandi¹ 

关键词: necroptosis;    apoptosis;    quercetin;    breast cancer;   

DOI  :  10.4149/BLL_2017_025

学科分类：医学（综合）

来源: AEPress, s.r.o.

【 摘 要 】

OBJECTIVE: This study investigated the quercetin (Que) effects on growth of MCF-7 human cancer breast cell line and its cellular death mechanism. BACKGROUND: Quercetin has been found to be very efficacious against many different types of cancer cells. However, the study is not sufficiently powered to demonastrate anticancer mechanisms.METHODS: MCF-7cells were treated by 50 µM/ ml of Que for 48 hours. MCF-7 cells were also pretreated with 10 Μm ZVAD (apoptosis inhibitor) or 3 mM Nec-1 (necroptosis inhibitor) for evaluation of cell death induced by apoptosis or necroptosis.RESULTS: MTT and clonogenicity assays revealed that the Que induced a significant increase in cell viability and proliferation in presence of Nec-1 in comparison to the presence of ZVAD (p < 0.05).

【 授权许可】

CC BY