PSidium guajava, a potential resource rich in corosolic acid revealed by high performance liquid chromatography

Dynamic Chiropractic

Qing-Wen Zhang¹  Song-Lin Li²  Ying Chen³  Yan Yi⁴ 

[1] 2The State Key Laboratory for Quality Research of Chinese Medicine, University of Macau, Macao SAR, China. 3Department of Metabolomics and Key Laboratory of New Drug Delivery System of Chinese, Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, 210028, China.;Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China. Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou, 510632, China.

关键词: Corosolic acid;    Psidium guajava;    high performance liquid chromatography (HPLC);    acidic hydrolysis.;   

DOI  :  

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Corosolic acid (CA) has become one of the most popular natural compounds in recent years because of its insulin-like and anti-cancer activities. In present study, a simple, precise and accurate high performance liquid chromatography method was developed to determine CA inPsidium guajava. An Agilent SB-C18 (4.6 × 250 mm, 5 μm) column was used for separation and analysis. The separation was performed with a constant mobile phase of acetonitrile and 0.2% formic acid in water (75:25) at a flow rate of 1 min/ml. The analytes were detected at the wavelength of 210 nm. The method was validated in terms of calibration curve, sensitivity, precision, accuracy and stability, and was applied to determine CA in leaves and fruits ofP. guajavafrom different locations. The results showed that CA was detected in leaves with the content almost 1%, but not detectable in fruits ofP. guajava. After the leaves were hydrolyzed with hydrochloric acid, the content of CA in leaf samples significantly increased up to 1.54%, and the increasing rates were more than 48.6%, suggesting that the leaf ofP. guajavais a potential resource rich in CA, and hydrochloric acid hydrolysis might be a cost-effective approach to produce CA from the leaf ofP. guajava.

【 授权许可】

Unknown