Streptococcus mutans Displays Altered Stress Responses While Enhancing Biofilm Formation by Lactobacillus casei in Mixed-Species Consortium

Frontiers in Cellular and Infection Microbiology

Li, Yihong¹  Jorgensen, Ashton²  Wen, Zezhang T.²  De, Arpan²  Feng, Shihai³  Liao, Sumei³  Xu, Xiaoming³  Caufield, Page W.³  Bitoun, Jacob P.⁴  Koo, Hyun⁵  Chain, Patrick S. G.⁵ 

[1] Basic Science and Craniofacial Biology, New York University College of Dentistry, United States;Center of Oral and Craniofacial Biology, Louisiana State University Health Sciences Center, United States;Department of Comprehensive Dentistry and Biomaterials, Louisiana State University Health Sciences Center, United States;Department of Microbiology, Immunology and Parasitology, Louisiana State University Health Sciences Center, United States;Genome Science Group, Bioscience Division, Los Alamos National Laboratory, United States

关键词: Streptococcus mutans;    lactobacilli;    interspecies interactions;    Biofilms;    Dental Caries;   

DOI  :  10.3389/fcimb.2017.00524

学科分类：生物科学（综合）

来源: Frontiers

【 摘 要 】

Like Streptococcus mutans, lactobacilli are commonly isolated from carious sites, although their exact role in caries development remains unclear. This study used mixed-species models to analyze biofilm formation by major groups of oral lactobacilli, including L. casei, L. fermentum, L. rhamnosus, L. salivarius ssp. salivarius and L. gasseri. The results showed that lactobacilli did not form good biofilms when grown alone, although differences existed between different species. When grown together with S. mutans, biofilm formation by L. gasseri and L. rhamnosus was increased by 2-log (P1-log (P1-log (P<0.001), but drastically reduced the survival rates following exposure to hydrogen peroxide (P<0.001), as compared to the respective mono-species cultures. When analyzed by RNA-seq, more than 134 genes were identified in S. mutans in dual-species with L. casei as either up- or down-regulated when compared to those grown alone. The up-regulated genes include those for superoxide dismutase, NADH oxidase, and members of the mutanobactin biosynthesis cluster. Among the down-regulated genes were those for GtfB and alternative sigma factor SigX. These results further suggest that interactions between S. mutans and oral lactobacilli are species-specific and may have significant impact on cariogenic potential of the community.

【 授权许可】

CC BY