Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection

Frontiers in Cellular and Infection Microbiology

Yamaguchi, Masaya¹  Honda-Ogawa, Mariko¹  Terao, Yutaka¹  Nakagawa, Seitaro²  Mori, Yasushi²  Matsue, Hiroyuki²  Sumitomo, Tomoko²  Kawabata, Shigetada²  Nakata, Masanobu³  Nakamura, Yuumi⁴ 

[1] Department of Dermatology, Chiba University Graduate School of Medicine, Japan;Department of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry, Japan;Division of Microbiology and Infectious Diseases, Niigata University Graduate School of Medical and Dental Sciences, Japan;Division of Special Care Dentistry, Osaka University Dental Hospital, Japan

关键词: Streptococcus pyogenes;    Epidermal barrier;    Cutaneous infection;    Desmogleins;    SpeB;   

DOI  :  10.3389/fcimb.2018.00010

学科分类：生物科学（综合）

来源: Frontiers

【 摘 要 】

Streptococcus pyogenes is responsible for a wide variety of cutaneous infections ranging from superficial impetigo to fulminant invasive necrotizing fasciitis. Dysfunction of desmosomes is associated with the pathogenesis of cutaneous diseases. We identified streptococcal pyrogenic exotoxin B (SpeB) as a proteolytic factor that cleaves the extracellular domains of desmoglein 1 and 3. In an epicutaneous infection model, lesional skin infected with an speB deletion mutant were significantly smaller as compared to those caused by the wild-type strain. Furthermore, immunohistological analysis indicated cleavage of desmogleins that developed around the invasion site of the wild-type strain. In contrast, the speB mutant was preferentially found on the epidermis surface layer. Taken together, our findings provide evidence that SpeB-mediated degradation of desmosomes has a pathogenic role in development of S. pyogenes cutaneous infection.

【 授权许可】

CC BY