Molecular diversity analysis of Asparagus racemosus and its adulterants using random amplified polymorphic DNA (RAPD)

Dynamic Chiropractic

Major Singh¹  Akansha Singh²  Ved Prakash Rai³ 

[1] Department of Floriculture and Landscaping, College of Horticulture, NDUAT, Kumarganj, Faizabad-224229, India.;Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, India.;Division of Crop Improvement, Indian Institute of Vegetable Research, Shahanshapur, Jakhini, Varanasi-221305, India.

关键词: Medicinal herb;    genetic diversity;    molecular marker;    authentication;    conservation.;   

DOI  :  10.5897/JMPR12.423

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Leaves of 28 genotypes of 4 species of the genusAsparagus,namely:Asparagus racemosus,Asparagus falcatus,Asparagus officinalisandAsparagus plumosuswere analyzed for genetic diversity by random amplified polymorphic DNA (RAPD). Out of one hundred 10-mer RAPD primers screened, 25 were selected for comparative analysis of different accessions ofA. racemosusand its closely related species. High genetic variations were found between the differentAsparagusspecies studied. Among a total of 296 RAPD fragments amplified, 287 bands (96.95%) were found polymorphic in the four species.Averagepolymorphic information content (PIC) value for the amplification product was 0.23.Fourteen species-specific bands were found inA. racemosusspecies.These species-specific RAPD markers could potentially be used for identifying authenticA. racemosusspp.andcould be sequenced for conversion to a sequence-characterized amplified region (SCAR) markerso as to serve as species specific marker.Jaccard’s genetic similarity co-efficient varied from0.19 to 0.85.Theunweighted pair group method with arithmetic average (UPGMA) dendrogram was constructed on the basis of Jaccard’s genetic similarity co-efficient valuesthat separate the three other species ofAsparagusfromA. racemosusaccessions.Results of this study will facilitateauthenticA. racemosusidentification, thereby aiding drug standardization, its collection, management and conservation.

【 授权许可】

Unknown