Effect of ultraviolet and ultrasonic on potential antidiabetic activity of in vitro shoot cultures of Orthosiphon aristatus

1st International Conference on Food and Agriculture 2018

Hunaefi, D.^1,2 ; Yuliana, N.D.^1 ; Smetanska, I.^3 ; Gruda, N.^4

Department of Food Science and Technology, Bogor Agricultural University (IPB), Indonesia^1

South East Asian Food, Agricultural Science and Technology Center, Bogor Agricultural University (IPB), Indonesia^2

University of Applied Science Weihenstephan, Triesdorf, Germany^3

University of Bonn, Germany^4

关键词: Alpha glucosidase;    Anti diabetics;    Antidiabetic activity;    Combined treatment;    Functional activities;    Medicinal herb;    Secondary metabolites;    Shoot cultures;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/207/1/012008/pdf
DOI  :  10.1088/1755-1315/207/1/012008

来源: IOP

【 摘 要 】

Orthosiphon aristatus Boldingh., a native tropical plant from Indonesia, is a medicinal herb that has been reported to possess an antidiabetic potential. However, study on this functional activity is limited especially on in vitro plant culture of Orthosiphon aristatus. The present study sought to investigate the effect of ultraviolet (UV) and ultrasonic (US) on this potential for in vitro shoot cultures of Orthosiphon aristatus (ISCOA). Methanol (70% v/v) extracts of ISCOA had the ability to inhibit alpha-amylase and alpha-glucosidase enzymes, dependent on the concentration of the extract. Among all treatment, highest inhibition of α-amylase and α-glucosidase activities were observed in combined treatment between UV for 60 minutes and US for 6 minutes. This was showed by the highest reduction by 0.4 point of IC50 inhibitory α-amylase and alpha-glucosidase activities in day 3 and in day 1 after exposure UV and US, respectively. The combination of UV and US has been shown to be effective in improving potential anti-diabetic properties of in vitro shoot cultures of Orthosiphon aristatus in which correlated with the increasing level of secondary metabolites.