The Escherichia coli Serogroup O1 and O2 Lipopolysaccharides Are Encoded by Multiple O-antigen Gene Clusters

Frontiers in Cellular and Infection Microbiology

Fach, Patrick¹  Beutin, Lothar²  phane⁴  Fleiss, Aubin⁵  Delannoy, Sabine⁸  Mariani-Kurkdjian, Patricia⁹  Bonacorsi, Sté^1,11 

[1] Escherichia coli, Service de Microbiologie, HôParis-Est, Maisons-Alfort, France;, Paris, France;CNR AssociéDepartment of Biology, Chemistry, Pharmacy, Institute for Biology - Microbiology, Freie UniversitäIAME, UMR 1137, INSERM, Paris, France;IAME, UMR 1137, University Paris Diderot, Sorbonne Paris CitéIdentyPath Platform, Food Safety Laboratory, Anses, UniversitéNational Reference Laboratory for Escherichia coli, Federal Institute for Risk Assessment (BfR), Berlin, Germany;pital Robert-Debrét Berlin, Berlin, Germany

关键词: Escherichia coli;    Extraintestinal escherichia coli;    ExPEC;    Shiga toxin-producing Escherichia coli;    STEC;    Serotyping;    O-antigen gene cluster;    O1;    O2;    O-antigen biosynthesis;   

DOI  :  10.3389/fcimb.2017.00030

学科分类：生物科学（综合）

来源: Frontiers

【 摘 要 】

E. coli strains belonging to serogroups O1 and O2 are frequently associated with human infections, especially extra-intestinal infections such as bloodstream infections or urinary tract infections. These strains can be associated with a large array of flagellar antigens. Because of their frequency and clinical importance, a reliable detection of E. coli O1 and O2 strains and also the frequently associated K1 capsule is important for diagnosis and source attribution of E. coli infections in humans and animals. By sequencing the O-antigen clusters of various O1 and O2 strains we showed that the serogroups O1 and O2 are encoded by different sets of O-antigen encoding genes and identified potentially new O-groups. We developed qPCR-assays to detect the various O1 and O2 variants and the K1-encoding gene. These qPCR assays proved to be 100% sensitive and 100% specific and could be valuable tools for the investigations of zoonotic and food-borne infection of humans with O1 and O2 extra-intestinal (ExPEC) or Shiga toxin-producing E. coli (STEC) strains.

【 授权许可】

CC BY