Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle

The Journal of Veterinary Medical Science

article

Kentaro OKUNO¹  Sharda Prasad AWASTHI¹  Germán A. KOPPRIO³  Atsushi IGUCHI⁴  Noritoshi HATANAKA¹  Atsushi HINENOYA¹  Rubén José LARA⁵  Shinji YAMASAKI¹ 

[1] Graduate School of Life and Environmental Sciences, Osaka Prefecture University;Asian Health Science Research Institute, Osaka Prefecture University;Leibniz-Institute of Freshwater Ecology and Inland Fisheries;Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki;National Council for Scientific and Technical Research, The Argentine Institute of Oceanography

关键词: Argentina;    beef cattle;    Escherichia coli;    enterohemorrhagic Escherichia coli;    Shiga toxin-producing Escherichia coli;   

DOI  :  10.1292/jvms.21-0002

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

The aims of this study were to investigate prevalence, O-genotype, and virulence gene profile including Shiga toxin (Stx) 2 gene-subtype of Stx-producing Escherichia coli (STEC) in beef cattle from the Bahía Blanca in Argentina. Rectal swabs were collected from 283 beef cattle in 2012. stx genes were detected in 90 (32%) out of the 283 rectal swabs by stx gene-specific PCR assay. The positive cases were 13 with stx1 , 58 with stx2, and 19 with both stx1 and stx2 . Among 90 stx gene-positive samples, 45 STEC strains were isolated, which included 3 stx1 , 34 stx2 , and eight stx1 and stx2 genes positive isolates. O-genotyping grouped 45 STEC strains into 19 different O-genotypes such as Og8, Og145, Og171, Og185 (4 from each), Og22, Og153, Og157 (3 from each) and others. Various stx2 gene-subtypes were identified in 42 STEC strains: 13 positive cases for stx2a , 11 for stx2c , 3 for stx2g , 10 for stx2a and stx2d , 4 for stx2a and stx2c , and 1 for stx2b , stx2c and stx2g . efaI gene, generally prevalent in clinical strains, was detected in relatively high in the STEC strains. These data suggest that stx2a and stx2c were distributed not only in O145 and O157 but also in minor O-genotypes of STEC in Argentina.

【 授权许可】

Unknown