A New Determinant of Candida glabrata Virulence: The Acetate Exporter CgDtr1

Frontiers in Cellular and Infection Microbiology

Romã¹  Fialho, Arsé¹  Santos, Rui¹  Cavalheiro, Mafalda²  Chibana, Hiroji²  Costa, Catarina²  Takahashi-Nakaguchi, Azusa³  Mil-Homens, Dalila³  Teixeira, Miguel C.³  o, Daniela⁴  nio M.⁴  Pais, Pedro⁴ 

[1] Biological Sciences Research Group, Department of Bioengineering, Instituto Superior TéInstitute for Bioengineering and Biosciences, Biological Sciences Research Group, Instituto Superior Técnico, Portugal;cnico, Universidade de Lisboa, Portugal

关键词: C;    ida glabrata;    CgDtr1;    Virulence;    Galleria mellonella;    Acetic acid resistance;   

DOI  :  10.3389/fcimb.2017.00473

学科分类：生物科学（综合）

来源: Frontiers

【 摘 要 】

Persistence and virulence of Candida glabrata infections are multifactorial phenomena, whose understanding is crucial to design more suitable therapeutic strategies. In this study, the putative multidrug transporter CgDtr1, encoded by ORF CAGL0M06281g, is identified as a determinant of C. glabrata virulence in the infection model Galleria mellonella. CgDTR1 deletion is shown to decrease the ability to kill G. mellonella larvae by decreasing C. glabrata ability to proliferate in G. mellonella hemolymph, and to tolerate the action of hemocytes. The possible role of CgDtr1 in the resistance to several stress factors that underlie death induced by phagocytosis was assessed. CgDTR1 was found to confer resistance to oxidative and acetic acid stress. Consistently, CgDtr1 was found to be a plasma membrane acetic acid exporter, relieving the stress induced upon C. glabrata cells within hemocytes, and thus enabling increased proliferation and virulence against G. mellonella larvae.

【 授权许可】

CC BY