Aerial parts of Solanum agrarium Sendtn. (Solanaceae) present the flavonoid myricetin 3,7,3 trimethyl ether and antispasmodic effect on guinea-pig ileum by blockade of voltage-gated calcium channels

Dynamic Chiropractic

im Macecirc⁵  Roberto Jefferson Bezerra do Nascimento⁷  Bagnolia Araujo da Silva⁹  Maria de Fatima Agra^1,12  Ana Carolina de Carvalho Correia^1,13  Celso de Amorim Camara^1,14  Rosimeire Ferreira dos Santos^1,15  Gislaine Alves de Oliveira^2,20  Tania Maria Sarmento da Silva^2,23  do^2,25  Ciberio L^2,28  Fabio de Souza Monteiro^2,29 

[1] Brazil.;PE –PI, Brazil.;Petrolina –ã, Campus Universitá, Universidade Federal da ParaíColegiado de Zootecnia, Universidade Federal do Vale do SãDepartamento de BioquíDepartamento de CiêDepartamento de QuíJoãPóba, P.O. Box 5009, 58051-970, de (CCS)”mica e Farmacologia, Universidade Federal do Piauímica, Universidade Federal Rural de Pernambuco, Campus Dois Irmãncias Farmacências Moleculares, Universidade Federal Rural de Pernambuco, Campus Dois Irmãncias da Saúnio Portella, CEP 64049-550 , Teresina –o Francisco, Rod. BR 407 Km 12 Lote 543 Projeto de Irrigaço Pessoa-PB, Brazil.;o Senador Nilo Coelho, s/no - C1. CEP 56300-000 –o em Produtos Naturais e Sintéos s/n, CEP 52171-980, Recife-PE, Brazil.;rio Ministro Petrôs-Graduaçticos Bioativos / Centro de Ciêuticas, Universidade Federal da Paraí

关键词: Solanum agrarium;    flavonoid;    antispasmodic effect;    guinea-pig ileum;    CaV-L.;   

DOI  :  10.5897/JMPR12.1165

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Solanum agrariumSendtn. is popularly known in Northeastern Brazil as "gogóia". Previous work has verified the presence of the flavonoid kaempferol and showed the crude ethanolic extract from aerial parts ofS. agrarium(SA‑EtOH) to have non-selective antispasmodic effect on guinea‑pig ileum. The aim was to isolate and identify the special metabolites and to investigate antispasmodic mechanism of SA‑EtOH on guinea-pig ileum. From their aerial parts was isolated the flavonoid myricetin‑3,7,3’‑trimethyl ether. SA‑EtOH shifted to the right cumulative concentration-response curves to carbachol or histamine in a reversible and non-competitive manner. SA‑EtOH relaxed the ileum pre-contracted with 40 mM KCl (EC50=17.4±3.5µg/ml), 10‑6M carbachol (EC50=119.4±24.3µg/ml) or 10‑6M histamine (EC50=18.7±4.6µg/ml). This is most likely due to the inhibition of calcium influx through voltage-gated calcium channels (CaV). It was confirmed by SA‑EtOH antagonizing CaCl2-induced contractions in depolarizing medium nominally without Ca2+, with non‑parallel shift of the concentration-response curves to the right and reduction of Emax. The uncovering of SA‑EtOH (EC50=187.2±27.3µg/ml) inhibiting tonic contractions induced by S‑(‑)‑BayK8644 suggests that Ca2+channel subtype involved is CaV-L. In conclusion, SA‑EtOH shows antispasmodic effect on guinea-pig ileum due, in part, to blockade of calcium influx through CaVchannels.

【 授权许可】

Unknown