Antioxidant, hepatoprotective, and ameliorative effects of Azadirachta indica on Eimeria papillata-induced infection in mice

Dynamic Chiropractic

Ahmed E. Abdel Moneim¹  Mohamed A. Dkhil² 

[1] Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt.;Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.

关键词: Neem;    liver;    Eimeria papillata;    oxidative stress;    mice.;   

DOI  :  10.5897/JMPR11.1778

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Azadirachta indica(neem) is used in traditional medicine for its pharmacological properties. Here, we investigated the effects of neem on the outcome of coccidiosis caused byEimeria papillatain mice. Coccidiosis was induced by infection of mice with 103sporulated oocysts ofE. papillata. The methanolic extract of neem (Azadirachta indica)leaves (500 mg/kg) was usedin vivofor its antioxidant and hepatoprotective properties. Anticoccidial and anti-inflammatory activities of neem were determined through oocyst output and liver histopathology, respectively. The data showed that mice infected withE. papillatarevealed an output of about 6.5×105oocysts per gram faeces on day 4 postinoculation (p.i.). This output is significantly decreased to about 2.7×105oocysts in neem-treated mice. Infection caused a marked liver injury as indicated by histopathological alterations as well as the significant increase in liver aspartate aminotransferase (AST), alanine aminotransferase, alkaline phosphatase (ALP) and bilurubin. Also, infection induced a significant increase in nitric oxide (NO) and malondialdehyde (MAD), and a significant decrease in glutathione (GSH). The methanolic extract ofA. indicaleaves showed a significant (P ≤ 0.05) hepatoprotective potential by improving the above mentioned parameters. Based on these results, it is concluded thatA. indicaleaves contain potent antioxidant compounds that could offer protection against hepatotoxicity as well as ameliorate preexisting liver damage and oxidative stress conditions.

【 授权许可】

Unknown