PLoS Pathogens | |
Age-Dependent Enterocyte Invasion and Microcolony Formation by Salmonella | |
Mark Kühnel1  Stefan Lienenklaus2  Siegfried Weiss2  Sara Leschner2  Melanie M. Brinkmann3  Michael Hensel4  Natalia Torow5  Marcus Fulde5  Mathias W. Hornef5  Aline Dupont5  Fredrik Gohde5  Kaiyi Zhang5  | |
[1] Centre for Anatomy, Hannover Medical School, Hannover, Germany;Department of Molecular Immunology, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany;Department of Viral Immune Modulation, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany;Division of Microbiology, University of Osnabrück, Osnabrück, Germany;Institute for Medical Microbiology and Hospital Epidemiology, Hannover Medical School, Hannover, Germany | |
关键词: Salmonella typhimurium; Salmonella; Salmonellosis; Gastrointestinal tract; Epithelial cells; Immunostaining; Gene expression; Spleen; | |
DOI : 10.1371/journal.ppat.1004385 | |
学科分类:生物科学(综合) | |
来源: Public Library of Science | |
【 摘 要 】
The coordinated action of a variety of virulence factors allows Salmonella enterica to invade epithelial cells and penetrate the mucosal barrier. The influence of the age-dependent maturation of the mucosal barrier for microbial pathogenesis has not been investigated. Here, we analyzed Salmonella infection of neonate mice after oral administration. In contrast to the situation in adult animals, we observed spontaneous colonization, massive invasion of enteroabsorptive cells, intraepithelial proliferation and the formation of large intraepithelial microcolonies. Mucosal translocation was dependent on enterocyte invasion in neonates in the absence of microfold (M) cells. It further resulted in potent innate immune stimulation in the absence of pronounced neutrophil-dominated pathology. Our results identify factors of age-dependent host susceptibility and provide important insight in the early steps of Salmonella infection in vivo. We also present a new small animal model amenable to genetic manipulation of the host for the analysis of the Salmonella enterocyte interaction in vivo.
【 授权许可】
CC BY
【 预 览 】
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