POLYETHYLENE GLYCOL EFFECT ON THE TRANSIENT AND STEADY STATE PHASES OF P-NITROPHENYLTRIMETHYL ACETATE HYDROLYSIS CATALYZED BY α-CHYMOTRYPSIN

Journal of The Chilean Chemical Society

LISSI, EDUARDO¹  CALDERÓN, CRISTIAN¹ 

[1] Universidad de Santiago de Chile, Chile

关键词: Enzyme kinetics;    chymotrypsin;    p-;    nitrophenyl trimethylacetate;    poly-;    ethyleneglycol.;   

DOI  :  10.4067/S0717-97072013000400033

学科分类：化学（综合）

来源: Sociedad Chilena de Quimica

【 摘 要 】

The effect of polyethylene glycol(PEG) on the hydrolytic activity of α-chymotrypsinhas been studied using p-nitrophenyltrimethyl acetate (PNTMA) as substrate.This substrate requires long times to reach the steady state condition, allowingthe measurement by conventional methods of the fast initial "burst" step presenton the biphasic catalytic behaviour exhibited by α-chymotrypsin.

【 授权许可】

Unknown