Potentiation of Epithelial Innate Host Responses by Intercellular Communication

PLoS Pathogens

Sanae Ben Mkaddem¹  Mathias W. Hornef¹  Tamas Dolowschiak²  Cécilia Chassin²  Alain Vandewalle³  Thilo M. Fuchs⁴  Siegfried Weiss⁵ 

[1] INSERM U773, Centre de Recherche Biomédicale Bichat-Beaujon, Paris, France;Institute of Medical Microbiology and Hospital Epidemiology, Hannover Medical School, Hannover, Germany;Molecular Immunology, Helmholtz Centre for Infection Research, Braunschweig, Germany;Université Paris 7-Denis Diderot, site Bichat, Paris, France;ZIEL, Department of Microbiology, Technical University Munich, Freising, Germany

关键词: Epithelial cells;    Listeria monocytogenes;    Listeria;    Flow cytometry;    Cell communication;    Cell cultures;    Gastrointestinal tract;    Small interfering RNAs;   

DOI  :  10.1371/journal.ppat.1001194

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

The epithelium efficiently attracts immune cells upon infection despite the low number of pathogenic microbes and moderate levels of secreted chemokines per cell. Here we examined whether horizontal intercellular communication between cells may contribute to a coordinated response of the epithelium. Listeria monocytogenes infection, transfection, and microinjection of individual cells within a polarized intestinal epithelial cell layer were performed and activation was determined at the single cell level by fluorescence microscopy and flow cytometry. Surprisingly, chemokine production after L. monocytogenes infection was primarily observed in non-infected epithelial cells despite invasion-dependent cell activation. Whereas horizontal communication was independent of gap junction formation, cytokine secretion, ion fluxes, or nitric oxide synthesis, NADPH oxidase (Nox) 4-dependent oxygen radical formation was required and sufficient to induce indirect epithelial cell activation. This is the first report to describe epithelial cell-cell communication in response to innate immune activation. Epithelial communication facilitates a coordinated infectious host defence at the very early stage of microbial infection.

【 授权许可】

CC BY