The Type III Secretion Effector NleE Inhibits NF-κB Activation

PLoS Pathogens

Thomas F. Meyer¹  Sina Bartfeld¹  Marganit Farago²  Yinon Ben-Neriah²  Irit Alkalay²  Ilan Rosenshine³  Chen Nadler³  Erez Mills³  Gili Haviv³  Kobi Baruch³  Simi Kobi³ 

[1] Department Molecular Biology, MPI for Infection Biology, Berlin, Germany;Department of Immunology and Cancer Research, IMRIC, Faculty of Medicine, The Hebrew University, Jerusalem, Israel;Department of Microbiology and Molecular Genetics, IMRIC, Faculty of Medicine, The Hebrew University, Jerusalem, Israel

关键词: Transcription factors;    Phosphorylation;    HeLa cells;    Cell staining;    Host cells;    Bacterial pathogens;    RNA extraction;    Secretion;   

DOI  :  10.1371/journal.ppat.1000743

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

The complex host-pathogen interplay involves the recognition of the pathogen by the host's innate immune system and countermeasures taken by the pathogen. Detection of invading bacteria by the host leads to rapid activation of the transcription factor NF-κB, followed by inflammation and eradication of the intruders. In response, some pathogens, including enteropathogenic Escherichia coli (EPEC), acquired means of blocking NF-κB activation. We show that inhibition of NF-κB activation by EPEC involves the injection of NleE into the host cell. Importantly, we show that NleE inhibits NF-κB activation by preventing activation of IKKβ and consequently the degradation of the NF-κB inhibitor, IκB. This NleE activity is enhanced by, but is not dependent on, a second injected effector, NleB. In conclusion, this study describes two effectors, NleB and NleE, with no similarity to other known proteins, used by pathogens to manipulate NF-κB signaling pathways.

【 授权许可】

CC BY