Biological research: BR | |
MiR-320 inhibits the growth of glioma cells through downregulating PBX3 | |
Yueran Zhao1  Ni Zheng2  Qi Gao2  Hua Gao2  Yuanquan Si2  Cuicui Pan2  | |
[1] Central Laboratory, Provincial Hospital Affiliated to Shandong University, Jinan, China;Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China | |
关键词: Glioma; Luciferase reporter assay; MiR-320; PBX3; | |
DOI : 10.1186/s40659-017-0137-4 | |
学科分类:生物科学(综合) | |
来源: BioMed Central | |
【 摘 要 】
MiR-320 is downregulated in multiple cancers, including glioma and acts as tumor suppressor through inhibiting tumor cells proliferation and inducing apoptosis. PBX3 (Pre-B cell leukemia homeobox 3), a putative target gene of miR-320, has been reported to be upregulated in various tumors and promote tumor cell growth through regulating MAKP/ERK pathway. This study aimed to verify whether miR-320 influences glioma cells growth through regulating PBX3. Twenty-four human glioma and paired adjacent nontumorous tissues were collected for determination of miR-320 and PBX3 expression using RT-qPCR and western blot assays. Luciferase reporter assay was performed to verify the interaction between miR-320 and its targeting sequence in the 3′ UTR of PBX3 in glioma cells U87 and U251. Increased miR-320 level in U87 and U251 cells was achieved through miR-320 mimic transfection and the effect of which on glioma cells growth, proliferation, cell cycle, apoptosis and activation of Raf-1/MAPK pathway was determined using MTT, colony formation, flow cytometry and western blot assays. PBX3 knockdown was performed using shPBX3 and the influence on MAPK pathway activation was evaluated. MiR-320 downregulation and PBX3 upregulation was found in glioma tissues. Luciferase reporter assays identified miR-320 directly blinds to the 3′ UTR of PBX3 in glioma cells. MiR-320 mimic transfection suppressed glioma cells proliferation, and induced cell cycle arrest and apoptosis. Both miR-320 overexpression and PBX3 knockdown inhibited Raf-1/MAPK activation. MiR-320 may suppress glioma cells growth and induced apoptosis through the PBX3/Raf-1/MAPK axis, and miR-320 oligonucleotides may be a potential cancer therapeutic for glioma.
【 授权许可】
CC BY
【 预 览 】
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RO201902015344837ZK.pdf | 1538KB | download |