Host Factor SAMHD1 Restricts DNA Viruses in Non-Dividing Myeloid Cells

PLoS Pathogens

Jessica Tate¹  Peter Gee¹  Yuka Kanemura²  Baek Kim²  Joseph A. Hollenbaugh²  Sarah M. Amie²  Michele B. Daly²  Natsumi Kasai²  Yoshio Koyanagi³  Dong-Hyun Kim⁴  Jonathon Baker⁴  Brian M. Ward⁴ 

[1] Center for Drug Discovery, The Department of Pediatrics, Emory University, Atlanta, Georgia, United States of America;Department of Microbiology and Immunology, University of Rochester, Rochester, New York, United States of America;Department of Pharmacy, Kyung-Hee University, Seoul, South Korea;Laboratory of Viral Pathogenesis, Institute for Virus Research, Kyoto University, Kyoto, Japan

关键词: Viral replication;    Vaccinia virus;    DNA replication;    Cell differentiation;    Macrophages;    dsDNA viruses;    Polymerase chain reaction;    Bone marrow cells;   

DOI  :  10.1371/journal.ppat.1003481

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

SAMHD1 is a newly identified anti-HIV host factor that has a dNTP triphosphohydrolase activity and depletes intracellular dNTP pools in non-dividing myeloid cells. Since DNA viruses utilize cellular dNTPs, we investigated whether SAMHD1 limits the replication of DNA viruses in non-dividing myeloid target cells. Indeed, two double stranded DNA viruses, vaccinia and herpes simplex virus type 1, are subject to SAMHD1 restriction in non-dividing target cells in a dNTP dependent manner. Using a thymidine kinase deficient strain of vaccinia virus, we demonstrate a greater restriction of viral replication in non-dividing cells expressing SAMHD1. Therefore, this study suggests that SAMHD1 is a potential innate anti-viral player that suppresses the replication of a wide range of DNA viruses, as well as retroviruses, which infect non-dividing myeloid cells.

【 授权许可】

CC BY