PLoS Pathogens | |
Damaged Intestinal Epithelial Integrity Linked to Microbial Translocation in Pathogenic Simian Immunodeficiency Virus Infections | |
Brian Tabb1  Jacob D. Estes1  Jeffrey Lifson1  Kenneth M. Oliveira2  Christopher J. Miller3  G. Robin Barclay4  Ashley T. Haase5  Mirko Paiardini6  Guido Silvestri6  Daniel C. Douek7  Levelle D. Harris8  Jason M. Brenchley8  Vanessa M. Hirsch8  Nichole R. Klatt8  Stefania Pittaluga9  Jeremy Smedley1,10  Rhonda Pung1,10  | |
[1] AIDS and Cancer Virus Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, Maryland, United States of America;AdvanDX, Inc., Woburn, Massachusetts, United States of America;Center for Comparative Medicine, California National Primate Research Center, University of California, Davis, California, United States of America;Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, Scotland;Department of Microbiology, Medical School, University of Minnesota, Minneapolis, Minnesota, United States of America;Department of Pathology and Laboratory of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America;Human Immunology Section, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, United States of America;Lab of Molecular Microbiology, NIAID, NIH, Bethesda, Maryland, United States of America;Lab of Pathology, NCI, NIH, Bethesda, Maryland, United States of America;Laboratory Animal Science Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, Maryland, United States of America | |
关键词: SIV; Colon; Immune activation; Macrophages; Genitourinary infections; Gastrointestinal tract; Opportunistic infections; T cells; | |
DOI : 10.1371/journal.ppat.1001052 | |
学科分类:生物科学(综合) | |
来源: Public Library of Science | |
【 摘 要 】
The chronic phase of HIV infection is marked by pathological activation of the immune system, the extent of which better predicts disease progression than either plasma viral load or CD4+ T cell count. Recently, translocation of microbial products from the gastrointestinal tract has been proposed as an underlying cause of this immune activation, based on indirect evidence including the detection of microbial products and specific immune responses in the plasma of chronically HIV-infected humans or SIV-infected Asian macaques. We analyzed tissues from SIV-infected rhesus macaques (RMs) to provide direct in situ evidence for translocation of microbial constituents from the lumen of the intestine into the lamina propria and to draining and peripheral lymph nodes and liver, accompanied by local immune responses in affected tissues. In chronically SIV-infected RMs this translocation is associated with breakdown of the integrity of the epithelial barrier of the gastrointestinal (GI) tract and apparent inability of lamina propria macrophages to effectively phagocytose translocated microbial constituents. By contrast, in the chronic phase of SIV infection in sooty mangabeys, we found no evidence of epithelial barrier breakdown, no increased microbial translocation and no pathological immune activation. Because immune activation is characteristic of the chronic phase of progressive HIV/SIV infections, these findings suggest that increased microbial translocation from the GI tract, in excess of capacity to clear the translocated microbial constituents, helps drive pathological immune activation. Novel therapeutic approaches to inhibit microbial translocation and/or attenuate chronic immune activation in HIV-infected individuals may complement treatments aimed at direct suppression of viral replication.
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201902014174010ZK.pdf | 4265KB | download |