DC-SIGN on B Lymphocytes Is Required For Transmission of HIV-1 to T Lymphocytes

PLoS Pathogens

Simon C Watkins¹  Todd A Reinhart²  David T Rowe²  Craig L Fuller²  Giovanna Rappocciolo²  Mariel Jais²  Phalguni Gupta²  Paolo Piazza²  Charles R Rinaldo Jr.³ 

[1] Department of Cell Biology and Physiology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America;Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America;Department of Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

关键词: B cells;    HIV-1;    T cells;    Flow cytometry;    Viral replication;    B cell receptors;    Tonsils;    T cell receptors;   

DOI  :  10.1371/journal.ppat.0020070

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Infection of T cells by HIV-1 can occur through binding of virus to dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) on dendritic cells and transfer of virus to CD4+ T cells. Here we show that a subset of B cells in the blood and tonsils of normal donors expressed DC-SIGN, and that this increased after stimulation in vitro with interleukin 4 and CD40 ligand, with enhanced expression of activation and co-stimulatory molecules CD23, CD58, CD80, and CD86, and CD22. The activated B cells captured and internalized X4 and R5 tropic strains of HIV-1, and mediated trans infection of T cells. Pretreatment of the B cells with anti–DC-SIGN monoclonal antibody blocked trans infection of T cells by both strains of HIV-1. These results indicate that DC-SIGN serves as a portal on B cells for HIV-1 infection of T cells in trans. Transmission of HIV-1 from B cells to T cells through this DC-SIGN pathway could be important in the pathogenesis of HIV-1 infection.

【 授权许可】

CC BY