Characterization of Reemerging Chikungunya Virus

PLoS Pathogens

Dominika Rudnicka¹  Felix A Rey¹  Philippe Desprès¹  Hafida Fsihi¹  Pierre-Emmanuel Ceccaldi¹  Ali Saïb¹  Fabien Blanchet¹  Clémentine Schilte¹  Matthew L Albert¹  Karin Le Roux¹  Nicoletta Casartelli²  Bruno Verhasselt³  Marie-Pascale Frenkiel⁴  Marie-Christine Prevost⁴  Alessia Zamborlini⁴  Philippe V Afonso⁴  Florence Guivel-Benhassine⁴  Nathalie Sol-Foulon⁴  Marion Sourisseau⁴  Fernando Arenzana-Seisdedos⁵  Alain Michault⁵  Olivier Schwartz⁵  Isabelle Schuffenecker⁶  Antoine Gessain⁷  Céline Trouillet⁸  Simona Ozden⁹ 

[1] CNRS URA 3015, Paris, France;Department of Immunology, Groupe Immunobiologie des Cellules Dendritiques, Institut Pasteur, Paris, France;Department of Virology, Unité Interactions Moléculaires Flavivirus-Hôtes, Institut Pasteur, Paris, France;Department of Virology, Unité Virus et Immunité, Institut Pasteur, Paris, France;Department of Virology, Unité d'Épidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur, Paris, France;Département Infection et Epidémiologie, Institut Pasteur, Paris, France;Département de Biologie Cellulaire et Infection, Plateforme de Microscopie Électronique, Institut Pasteur, Paris, France;INSERM U818, Paris, France;Laboratoire de Microbiologie, Groupe Hospitalier Sud Réunion, Ile de la Réunion, France

关键词: Chikungunya virus;    Chikungunya infection;    HeLa cells;    Viral replication;    Flow cytometry;    Macrophages;    Cell staining;    Antibodies;   

DOI  :  10.1371/journal.ppat.0030089

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

An unprecedented epidemic of chikungunya virus (CHIKV) infection recently started in countries of the Indian Ocean area, causing an acute and painful syndrome with strong fever, asthenia, skin rash, polyarthritis, and lethal cases of encephalitis. The basis for chikungunya disease and the tropism of CHIKV remain unknown. Here, we describe the replication characteristics of recent clinical CHIKV strains. Human epithelial and endothelial cells, primary fibroblasts and, to a lesser extent, monocyte-derived macrophages, were susceptible to infection and allowed viral production. In contrast, CHIKV did not replicate in lymphoid and monocytoid cell lines, primary lymphocytes and monocytes, or monocyte-derived dendritic cells. CHIKV replication was cytopathic and associated with an induction of apoptosis in infected cells. Chloroquine, bafilomycin-A1, and short hairpin RNAs against dynamin-2 inhibited viral production, indicating that viral entry occurs through pH-dependent endocytosis. CHIKV was highly sensitive to the antiviral activity of type I and II interferons. These results provide a general insight into the interaction between CHIKV and its mammalian host.

【 授权许可】

CC BY