Biological research: BR | |
Cadmium-induced apoptosis of Siberian tiger fibroblasts via disrupted intracellular homeostasis | |
Weijun Guan1  Ziao Yuan2  Zheng Liu2  Xueting Liu2  Chunwen Yang2  Hongyi Yuan2  Wenxiu Zhang3  Hui Wang3  | |
[1] College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang, China;Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China;Jinzhou Medical University, Jinzhou, China | |
关键词: Siberian tiger; Cadmium; Apoptosis; Fibroblast; Calcium; | |
DOI : 10.1186/s40659-016-0103-6 | |
学科分类:生物科学(综合) | |
来源: BioMed Central | |
【 摘 要 】
Heavy metals can cause great harm to Siberian tigers in the natural environment. Cadmium (Cd2+) is an environmental contaminant that affects multiple cellular processes, including cell proliferation, differentiation, and survival. It has been shown to induce apoptosis in a variety of cell types and tissues. We investigated the apoptotic effects of Cd2+ on Siberian tiger fibroblasts in vitro. Our research revealed the typical signs of apoptosis after Cd2+ exposure. Apoptosis was dose- (0–4.8 μM) and duration-dependent (12–48 h), and proliferation was strongly inhibited. Cd2+ increased the activity of caspase-3, -8, and -9 and disrupted calcium homeostasis by causing oxidative stress and mitochondrial dysfunction. It also increased K+ efflux and altered the mRNA levels of Bax, Bcl-2, caspase-3, caspase-8, Fas, and p53. Our results suggest that Cd2+ triggers the apoptosis of Siberian tiger fibroblasts by disturbing intracellular homeostasis. These results will aid in our understanding of the effects of Cd2+ on Siberian tigers and in developing interventions to treat and prevent cadmium poisoning.
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201902011567649ZK.pdf | 5884KB | download |