Development and validation of a method for the analysis of Ochratoxin A in roasted coffee by liquid chromatography/electrospray-mass spectrometry in Tandem (LC/ESI-MS/MS)

Quimica nova

de la Cruz, Marcus Henrique C.¹  Godoy, Ronoel Luiz O.²  Uekane, Thais M.²  Cunha, Valnei S.²  Cunha, Carolina P. da²  Caixeiro, Janaina M. R.²  Geaquinto, Luths R. O.²  Bandeira, Raquel D. C. C.³ 

[1] Embrapa Agroindústria de Alimentos, Rio de Janeiro, Brasil;Instituto Nacional de Metrologia Normalização e Qualidade Industrial, Duque de Caxias, Brasil;Universidade Federal Rural do Rio de Janeiro, Seropédica, Brasil

关键词: coffee;    Ochratoxin A;    LC/ESI-MS/MS.;   

DOI  :  10.1590/S0100-40422012000100013

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A method using LC/ESI-MS/MS for the quantitative analysis of Ochratoxin A in roasted coffee was described. Linearity was demonstrated (r = 0.9175). The limits of detection and quantification were 1.0 and 3.0 ng g-1, respectively. Trueness, repeatability and intermediate precision values were 89.0-108.8%; 2.4-13.7%; 12.5-17.8%, respectively. To the best of our knowledge, this is the first report in which Ochratoxin A in roasted coffee is analysed by LC/ESI-MS/MS, contributing to the field of mycotoxin analysis, and it will be used for future production of Certified Reference Material.

【 授权许可】

CC BY