Development and validation of a method for the analysis of Ochratoxin A in roasted coffee by liquid chromatography/electrospray-mass spectrometry in Tandem (LC/ESI-MS/MS)

Química Nova

Raquel D. C. C. Bandeira²  Thais M. Uekane¹  Carolina P. Da Cunha¹  Luths R. O. Geaquinto¹  Valnei S. Cunha¹  Janaina M. R. Caixeiro¹  Ronoel Luiz O. Godoy¹  Marcus Henrique C. De La Cruz¹ 

[1] ,Universidade Federal Rural do Rio de Janeiro Instituto de Tecnologia Seropédica RJ ,Brasil

关键词: coffee;    Ochratoxin A;    LC/ESI-MS/MS;   

DOI  :  10.1590/S0100-40422012000100013

来源: SciELO

【 摘 要 】

A method using LC/ESI-MS/MS for the quantitative analysis of Ochratoxin A in roasted coffee was described. Linearity was demonstrated (r = 0.9175). The limits of detection and quantification were 1.0 and 3.0 ng g-1, respectively. Trueness, repeatability and intermediate precision values were 89.0-108.8%; 2.4-13.7%; 12.5-17.8%, respectively. To the best of our knowledge, this is the first report in which Ochratoxin A in roasted coffee is analysed by LC/ESI-MS/MS, contributing to the field of mycotoxin analysis, and it will be used for future production of Certified Reference Material.

【 授权许可】

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