Chondroitin sulfate activates B cells in vitro, expands CD138+ cells in vivo, and interferes with established humoral immune responses

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: glycosaminoglycans;    plasma cells;    collagen‐;    induced arthritis;   

DOI  :  10.1189/jlb.1A0913-502R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Glycosaminoglycanshaveanti‐inflammatorypropertiesandinteractwithavarietyofsolubleandmembrane‐boundmolecules.LittleisknownabouttheireffectsonBcellsandhumoralimmuneresponses.WeshowthatCSbutnotdextranorotherglycosaminoglycansinducesapronouncedproliferationofBcellsinvitrocomparedwithTLR4orTLR9ligands.WiththeuseofinhibitorsandKOmice,wedemonstratethatthisproliferationismediatedbythetyrosinekinasesBTKandSykbutindependentofCD44.AntibodiesagainstIg‐αorIg‐βcompletelyblockCS‐inducedBcellproliferation.InjectionofCSinmicefor4–5daysexpandsBcellsinthespleenandresultsinamarkedincreaseofCD138+cellsinthespleenthatisdependentonBTKbutindependentofCD4+Tcells.Long‐termtreatmentwithCSfor14daysalsoincreasesCD138+cellsinthebonemarrow.WhenmicewereimmunizedwithAPCorcollagenandtreatedwithCSforupto14daysduringprimaryoraftersecondaryimmuneresponses,antigen‐specifichumoralimmuneresponsesandantigen‐specificCD138+plasmacellsinthebonemarrowwerereducedsignificantly.ThesedatashowthatCD138+cells,inducedbytreatmentwithCS,migrateintothebonemarrowandmaydisplaceotherantigen‐specificplasmacells.Overall,CSisabletointerferemarkedlywithprimaryandfullyestablishedhumoralimmuneresponsesinmice...

【 授权许可】

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