Chronic β-adrenoceptor Antagonists Upregulate the Rat Alveolar Macrophage Adrenergic System Through the β1-Subtype

Cellular Physiology and Biochemistry

关键词: β1-adrenergic receptor;    Propranolol;    Atenolol;    Alveolar macrophages;   

DOI  :  10.1159/000331747

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Background Previous studies demonstrate that macrophages synthesis and release catecholamines, which regulate the immune responses in an autocrine manner. These responses are mediated in part by β-adrenoceptors expressed on macrophages. Some β-adrenoceptor antagonists are commonly used in clinical conditions. Here we investigated whether the chronic administration of β-adrenoceptor antagonists upregulate adrenergic system of alveolar macrophage and the potential mechanims. Methods Propranolol (30 mg/kg·d) or atenolol (5 mg/kg·d) was administered by gavage to rats for 4 weeks. Then alveolar macrophages were isolated and the expression of β₁ or β₂-adrenoceptor was detected by flow cytometric analysis. Dopamine β-hydroxylase expression was assessed by Western blot assay and the concentrations of noradrenaline, IL-6, and TNF-α in cell supernatants were measured using ELISA after 2 h or 24 h exposure of alveolar macrophages to 100 ng/ml lipopolysaccharide (LPS). Results Propranolol increased the mean fluorescence intensity (MFI) of β₁, β₂-adrenoceptor and the frequency of β₁-,β₂- adrenoceptor positive macrophages. However, only the MFI of β₁-adrenoceptor and the frequency of β₁-adrenoceptor positive macrophages were increased by atenolol. Furthermore, both propranolol and atenolol promoted LPS-mediated dopamine β-hydroxylase protein expression and increased noradrenaline production in rat alveolar macrophages. This was accompanied by increased LPS-mediated IL-6 and TNF-α production in cell supernatants of alveolar macrophages. Conclusion These findings demonstrate that propranolol or atenolol upregulates alveolar macrophage adrenergic system, and the response may be β₁-adrenergic receptor subtype dependent.

【 授权许可】

CC BY-NC-ND