Surface changes and polymyxin interactions with a resistant strain of Klebsiella pneumoniae:

Innate Immunity

TonyVelkov¹ 

关键词: Polymyxin;    colistin;    Klebsiella pneumoniae;    lipopolysaccharide;    surface;   

DOI  :  10.1177/1753425913493337

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

This study examines the interaction of polymyxin B and colistin with the surface and outer membrane components of a susceptible and resistant strain of Klebsiella pneumoniae. The interaction between polymyxins and bacterial membrane and isolated LPS from paired wild type and polymyxin-resistant strains of K. pneumoniae were examined with N-phenyl-1-naphthylamine (NPN) uptake, fluorometric binding and thermal shift assays, lysozyme and deoxycholate sensitivity assays, and by 1H NMR. LPS from the polymyxin-resistant strain displayed a reduced binding affinity for polymyxins B and colistin in comparison with the wild type LPS. The outer membrane NPN permeability of the resistant strain was greater compared with the susceptible strain. Polymyxin exposure enhanced the permeability of the outer membrane of the wild type strain to lysozyme and deoxycholate, whereas polymyxin concentrations up to 32 mg/ml failed to permeabilize the outer membrane of the resistant strain. Zeta potential measurements revealed that ...

【 授权许可】

CC BY