Autophagy counters LPS-mediated suppression of lysozyme:

Innate Immunity

Sudha BSingh¹ 

关键词: Autophagy;    Desulfovibrio vulgaris;    lipopolysaccharide;    lysozyme;    rapamycin;   

DOI  :  10.1177/1753425917721630

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Impaired Paneth cell expression of antimicrobial protein (AMP) lysozyme is found in patients with Crohn’s disease with the autophagy gene ATG16L1 risk allele, in mice with mutations in autophagy genes Atg16L1, Atg5 and Atg7, and in Irgm1 knockout mice. Defective autophagy is also associated with expansion of resident Gram-negative bacteria in the intestinal lumen. These findings suggest that autophagy may control extracellular resident microbes by governing expression of lysozyme. To test the hypothesis that autophagy may have a defensive role in host response to resident extracellular microbes, we investigated the relationship between gut microbes, autophagy, and lysozyme. RAW 264.7 macrophages were treated with fecal slurry (FS), representing the resident microbial community; lipopolysaccharide (LPS); or butyrate, representing microbial products; or a representative resident Gram-negative bacterium Desulfovibrio vulgaris (DSV). FS, LPS, and DSV inhibited lysozyme expression, whereas butyrate had no effe...

【 授权许可】

CC BY