The Compromised Anti-microbial Function of Mesenchymal Stem Cells in Diabetic Patients:

Foot & Ankle Orthopaedics

ZijunZhang¹ 

关键词: diabetes;    mesenchymal stem cells;    bacteria;    infection;   

DOI  :  10.1177/2473011417S000434

学科分类：医学（综合）

来源: Sage Journals

【 摘 要 】

Category:Basic Sciences/BiologicsIntroduction/Purpose:Diabetic foot infection (DFI), including skin infection and osteomyelitis, is a severe complication of late- stage diabetes. Mesenchymal stem cells (MSCs) facilitate bacterial clearance. In bacterial infection, MSCs, via paracrine mediators, regulate the host cell metabolism and inflammatory response. Particularly, MSCs augment the antibacterial function of neutrophils. It is generally believed that hyperglycemia in diabetes is toxic to MSCs/progenitors and detrimental to their regenerative function. It is unknown, however, whether the antibacterial function of MSCs is compromised in diabetes.Methods:Bone marrow samples from 6 diabetic and 4 non-diabetic patients (approved by IRB) were used for MSC isolation.1. MSCs from both diabetic and non-diabetic patients were treated with lipopolysaccharides (LPS), a bacterial wall component, for 6 hours. The tissue culture media were collected as conditioned medium. E. coli from a single colony were cultured wit...

【 授权许可】

CC BY