Genotype identification of Enterocytozoon bieneusi isolates from stool samples of HIV-infected Tunisian patients

Parasite

and K. Aoun¹  A. Bouratbine¹  M. Thellier³  F. Kanoun²  J. Breton³  R. Abdelmalek²  N. Chabchoub¹ 

[1] Laboratoire de recherche “Parasitoses médicales, biotechnologie et bio-molécules” LR 11-IPT-06 & Laboratoire de parasitologie, Institut Pasteur de Tunis,Tunisie;Service des maladies infectieuses, Hôpital de la Rabta,Tunis,Tunisie;Hôpital de la Pitié-Salpétrière, Unité INSERM 511, Université Paris VI Pierre et Marie Curie,Paris,France

关键词: Tunisia;    HIV;    Internal Transcribed Spacer;    genotype;    Enterocytozoon bieneusi;    microsporidia;   

Others  :  808819
DOI  :  doi:10.1051/parasite/2012192147

 received in 2011-08-04, accepted in 2011-12-04,  发布年份 2012

【 摘 要 】

The microsporidian species Enterocytozoon bieneusi is a major cause of chronic diarrhea and malabsorption in patients with AIDS. Genotyping was performed on seven E. bieneusi strains for the first time in Tunisia. All the strains were isolated from stool samples of humans with immunodeficiency virus (HIV) infection. Analysis of the ribosomal RNA gene internal transcribed spacer (rDNA ITS) allowed the identification of three distinct genotypes previously described in other studies. Genotypes D and B were characterized in four and two respectively. The Peruvian genotype (Peru 8) was detected in the last isolate. These results indicate a genetic diversity in E. bieneusi strains from HIV Tunisian patients and suggest the coexistence of both zoonotic and anthroponotic route of transmission.

【 授权许可】

   
© PRINCEPS Editions, Paris, 2012

【 图 表 】

Fig. 1.

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