Mitochondrial DNA alteration in obstructive sleep apnea

Respiratory Research

Maria P Foschino Barbaro¹  Roberto Sabato¹  Giulia Patricelli¹  Grazia P Palladino¹  Grazia Cotugno¹  Elisabetta Crisetti¹  Giovanna E Carpagnano¹  Donato Lacedonia¹ 

[1] Department of Medical and Surgical Sciences, Institute of Respiratory Diseases, University of Foggia, Viale degli Aviatori, Foggia 71100, Italy

关键词: ROMs;    Oxidative stress;    OSAS;    Mitochondrial DNA;   

Others  :  1175085
DOI  :  10.1186/s12931-015-0205-7

 received in 2014-10-22, accepted in 2015-03-13,  发布年份 2015

【 摘 要 】

Background

Obstructive Sleep Apnea (OSAS) is a disease associated with the increase of cardiovascular risk and it is characterized by repeated episodes of Intermittent Hypoxia (IH) which inducing oxidative stress and systemic inflammation. Mitochondria are cell organelles involved in the respiratory that have their own DNA (MtDNA). The aim of this study was to investigate if the increase of oxidative stress in OSAS patients can induce also MtDNA alterations.

Methods

46 OSAS patients (age 59.27 ± 11.38; BMI 30.84 ± 3.64; AHI 36.63 ± 24.18) were compared with 36 control subjects (age 54.42 ± 6.63; BMI 29.06 ± 4.7; AHI 3.8 ± 1.10). In blood cells Content of MtDNA and nuclear DNA (nDNA) was measured in OSAS patients by Real Time PCR. The ratio between MtDNA/nDNA was then calculated. Presence of oxidative stress was evaluated by levels of Reactive Oxygen Metabolites (ROMs), measured by diacron reactive oxygen metabolite test (d-ROM test).

Results

MtDNA/nDNA was higher in patients with OSAS than in the control group (150.94 ± 49.14 vs 128.96 ± 45.8; p = 0.04), the levels of ROMs were also higher in OSAS subjects (329.71 ± 70.17 vs 226 ± 36.76; p = 0.04) and they were positively correlated with MtDNA/nDNA (R = 0.5, p < 0.01).

Conclusions

In OSAS patients there is a Mitochondrial DNA damage induced by the increase of oxidative stress. Intermittent hypoxia seems to be the main mechanism which leads to this process.

【 授权许可】

   
2015 Lacedonia et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

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