Molecular characterization of cryptic species of Anopheles barbirostris van der Wulp in China

Particle and Fibre Toxicology

Yajun Ma²  Jiannong Xu²  Yan Wang¹ 

[1] Department of Tropical Infectious Diseases, Second Military Medical University, 800 Xiangyin Rd, Shanghai 200433, China;Haikou Center for Disease Control and Prevention, China CDC Key Laboratory of Surveillance and Early- Warning on Infectious Disease, 20 Yehai Rd, Haikou, Hainan 571100, China

关键词: China;    Species complex;    Anopheles barbirostris;   

Others  :  1148445
DOI  :  10.1186/s13071-014-0592-5

 received in 2014-09-27, accepted in 2014-12-06,  发布年份 2014

【 摘 要 】

Background

Anopheles barbirostris sensu lato belongs to the Barbirostris subgroup of the subgenus Anopheles that is distributed in Southeast Asia. Different molecular forms have been identified based on the rDNA-ITS2 and mtDNA-COI sequences. Anopheles barbirostris occurs in China. The species status was uncertain due to the lack of molecular characterization. The present study characterized Chinese An. barbirostris using rDNA-ITS2 and mtDNA-COI gene sequences. Two cryptic species were identified.

Findings

DNA was extracted from morphologically identified An. barbirostris specimens collected in Yunnan and Hainan from China, the sequences of rDNA-ITS2 and mtDNA-COI regions of 40 individuals were amplified and analyzed. The sequence comparison revealed two cryptic species, corresponding to An. barbirostris A1/clade III and A2/clade IV, respectively. The molecular characterization updated the species composition of the An. barbirostris complex in China.

Conclusions

This study distinguished two molecular forms in the An. barbirostris s. l. in China.

【 授权许可】

   
2014 Wang et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

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