Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka

Particle and Fibre Toxicology

Sinnathamby N Surendran¹  Ranjan Ramasamy¹  Lalanthika BS Peiris³  Sara L Goodacre²  Pavilupillai J Jude¹  Kanapathy Gajapathy¹ 

[1] Department of Zoology, Faculty of Science, University of Jaffna, Jaffna 40000, Sri Lanka;School of Life Sciences, University of Nottingham, Nottingham NG7 2RD, UK;Regional Office, Anti Malaria Campaign, Hambantota 82000, Sri Lanka

关键词: Sri Lanka;    Species complex;    Malaria vector;    Anopheles barbirostris;   

Others  :  1181888
DOI  :  10.1186/1756-3305-7-348

 received in 2014-04-10, accepted in 2014-07-02,  发布年份 2014

【 摘 要 】

Background

Anopheles barbirostris is a vector of malaria in Sri Lanka. The taxon exists as a species complex in the Southeast Asian region. Previous studies using molecular markers suggest that there are more than 4 distinct clades within the An. barbirostris complex in Southeast Asia. The present study characterizes Sri Lankan An. barbirostris using mtDNA cytochrome oxidase subunit I (COI) and ribosomal RNA internal transcribed spacer 2 (ITS2) gene sequences.

Findings

DNA was extracted from morphologically identified An. barbirostris specimens from Sri Lanka, the COI and ITS2 regions amplified and their sequences analysed by comparison with other GenBank entries. Maximum likelihood trees suggested that Sri Lankan An. barbirostris constitute a different molecular type most closely related to clade I.

Conclusions

Considering the uncorrected p distances between the clade I and Sri Lankan specimens it is fair to assume that the specimens collected from widely separated locations in Sri Lanka with morphology characteristic of An. barbirostris s.l. form a new molecular type with close resemblance to An. barbirostris s.s from Indonesia and Thailand.

【 授权许可】

   
2014 Gajapathy et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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