Rapeseed and milk protein exhibit a similar overall nutritional value but marked difference in postprandial regional nitrogen utilization in rats

Nutrition & Metabolism

Cécile Bos¹  Daniel Tomé¹  François Blachier¹  François Mariotti¹  Hélène Fouillet¹  Claire Boutry¹ 

[1] AgroParisTech, CNRH-IdF, UMR914 Nutrition Physiology and Ingestive Behavior, F-75005 Paris, France

关键词: tissue protein synthesis;    nutritional quality;    postprandial metabolism;    dietary protein;   

Others  :  820994
DOI  :  10.1186/1743-7075-8-52

 received in 2011-03-29, accepted in 2011-07-25,  发布年份 2011

【 摘 要 】

Background

Rapeseed is an emerging and promising source of dietary protein for human nutrition and health. We previously found that rapeseed protein displayed atypical nutritional properties in humans, characterized by low bioavailability and a high postprandial biological value. The objective of the present study was to investigate the metabolic fate of rapeseed protein isolate (RPI) and its effect on protein fractional synthesis rates (FSR) in various tissues when compared to a milk protein isolate (MPI).

Methods

Rats (n = 48) were given a RPI or MPI meal, either for the first time or after 2-week adaptation to a MPI or RPI-based diet. They were divided in two groups for measuring the fed-state tissue FSR 2 h after the meal (using a flooding dose of ¹³C-valine) and the dietary N postprandial distribution at 5 h (using ¹⁵N-labeled meals).

Results

RPI and MPI led to similar FSR and dietary nitrogen (N) losses (ileal and deamination losses of 4% and 12% of the meal, respectively). By contrast, the dietary N incorporation was significantly higher in the intestinal mucosa and liver (+36% and +16%, respectively) and lower in skin (-24%) after RPI than MPI.

Conclusions

Although RPI and MPI led to the same overall level of postprandial dietary N retention in rats (in line with our findings in humans), this global response conceals marked qualitative differences at the tissue level regarding dietary N accretion. The fact that FSR did not however differed between groups suggest a differential modulation of proteolysis after RPI or MPI ingestion, or other mechanisms that warrant further study.

【 授权许可】

   
2011 Boutryet al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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