Toxoplasma gondii sexual cross in a single naturally infected feline host: Generation of highly mouse-virulent and avirulent clones, genotypically different from clonal types I, II and III

Veterinary Research

Gereon Schares²  Franz J Conraths²  Majda G Vrhovec¹  Nikola Pantchev¹  Aline Maksimov²  Andrea Bärwald²  Daland C Herrmann² 

[1] VetMed Labor GmbH, Division of IDEXX Laboratories, Mörikestr. 28/3, 71636, Ludwigsburg, Germany;Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestr. 55, 16868, Wusterhausen, Germany

关键词: Virulence;    Cats;    PCR-RFLP;    Genotype;    Toxoplasma gondii;   

Others  :  817314
DOI  :  10.1186/1297-9716-43-39

 received in 2011-11-11, accepted in 2012-03-13,  发布年份 2012

【 摘 要 】

Tachyzoite clones obtained from a single Toxoplasma gondii oocyst field sample were genotyped and characterized regarding mouse virulence. PCR-RFLP genotyping of tachyzoites initially isolated from interferon-γ-knockout (GKO) mice, BALB/c mice and VERO cell culture using the nine independent, unlinked genetic markers nSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico revealed mixed T. gondii infections showing combinations of type II and type III alleles at different loci. Forty-five individual clones were obtained from all mixed T. gondii tachyzoite cell cultures by limiting dilution. Sixteen T. gondii clones showed type III alleles at all loci and 29 clones displayed a combination of type II and type III alleles at different loci. Five clone groups were identified in total, four of which include T. gondii clones that showed a non-canonical allele pattern and have never been described in natural infections before. All tested clones, except two, were highly virulent in BALB/c mice. The isolation of different non-canonical T. gondii clones originating from an oocyst sample of a single naturally infected cat demonstrate that sexual recombination as well as re-assortment of chromosomes via a sexual cross of T. gondii occur under natural conditions and result in the emergence of clones with increased virulence in mice.

【 授权许可】

   
2012 Herrmann et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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