Infectious alphavirus production from a simple plasmid transfection

Virology Journal

Brian J Geiss²  Ken E Olson¹  Siddhi BC Lama¹  Brittney R Henderson¹  J Jordan Steel¹ 

[1] Department of Microbiology, Immunology, and Pathology, 1682 Campus Delivery, Colorado State University, Fort Collins, CO 80523 USA;Department of Biochemistry and Molecular Biology, 1870 Campus Delivery, Colorado State University, Fort Collins, CO 80523 USA

关键词: Gateway;    Virus production;    Alphavirus;    Sindbis;   

Others  :  1156431
DOI  :  10.1186/1743-422X-8-356

 received in 2011-03-25, accepted in 2011-07-19,  发布年份 2011

【 摘 要 】

We have developed a new method for producing infectious double subgenomic alphaviruses from plasmids transfected into mammalian cells. A double subgenomic Sindbis virus (TE3'2J) was transcribed from a cytomegalovirus PolII promoter, which results in the production of infectious virus. Transfection of as little as 125 ng of plasmid is able to produce 1 × 10⁸ plaque forming units/ml (PFU/ml) of infectious virus 48 hours post-transfection. This system represents a more efficient method for producing recombinant Sindbis viruses.

【 授权许可】

   
2011 Steel et al; licensee BioMed Central Ltd.

【 图 表 】

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