Human immunodeficiency virus‐1 Nef protein interacts with Tat and enhances HIV‐1 gene expression

FEBS Letters

Joseph, Ajith Mathew¹  Ladha, Jayashree S¹  Mojamdar, Manoj¹  Mitra, Debashis¹ 

[1] National Centre for Cell Science, Ganeshkhind, Pune 411007, India

关键词: Human immunodeficiency virus;    Nef;    Tat;    Replication;    Expression;    Virus production;   

DOI  :  10.1016/S0014-5793(03)00725-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The human immunodeficiency virus (HIV-1) Nef protein is now regarded as a regulatory protein responsible not only for establishment of infection and increased pathogenesis but also for enhancement of viral replication. However, the mechanism of Nef-induced activation of viral replication remains to be clearly understood. Using transient transfection assay, co-immunoprecipitation and pull-down analysis, we demonstrate in this report that the HIV-1 Nef protein physically interacts with Tat, the principal transactivating protein of HIV-1. Our observations with single cycle replication experiments further indicate that this interaction results not only in enhancement of Tat-induced HIV-1 long terminal repeat-mediated gene expression but also in virus production.

【 授权许可】

Unknown