Genetic characterization of Toxoplasma gondii from Qinghai vole, Plateau pika and Tibetan ground-tit on the Qinghai-Tibet Plateau, China

Particle and Fibre Toxicology

Xing-Quan Zhu¹  Chunlei Su²  Wan-Zhong Jia³  Dong-Hui Zhou³  Si-Yang Huang³  Zhong-Zi Lou³  Xiao-Xuan Zhang⁴ 

[1] College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China;Department of Microbiology, The University of Tennessee, Knoxville, TN 37996, USA;State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China;Department of Veterinary Medicine, College of Agriculture, Yanbian University, Yanji, Jilin Province 133000, PR China

关键词: Qinghai-Tibet Plateau;    Wildlife;    PCR-RFLP;    Genetic typing;    Toxoplasma gondii;   

Others  :  824329
DOI  :  10.1186/1756-3305-6-291

 received in 2013-09-15, accepted in 2013-10-07,  发布年份 2013

【 摘 要 】

Background

The distribution of genetic diversity of Toxoplasma gondii in wildlife is of interest to understand the transmission of this parasite in the environment. Limited information on T. gondii genotypes has been reported in wildlife in China. The objective of this study was to carry out the genetic characterization of T. gondii isolates from wild animals on the Qinghai-Tibet Plateau.

Methods

Using PCR and multilocous polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology, we detected genetic diversity of T. gondii isolates from Qinghai vole, Plateau pika and Tibetan ground-tit in these regions.

Results

In total, 183 brain tissues of different wild animals, including 48 Qinghai vole (Microtus fuscus), 101 Plateau pika (Ochotona curzoniae) and 34 Tibetan ground-tit (Pseudopodoces humilis), were tested for T. gondii infection. 11 of these were found to be positive for the T. gondii B1 gene by PCR amplification. These positive DNA samples were typed at 10 genetic markers, including 9 nuclear loci (SAG1, 5’-and 3’-SAG2, alternative SAG2, BTUB, GRA6, L358, PK1, c22-8, c29-2), and an apicoplast locus Apico. Six were successfully genotyped at eight or more genetic loci, and were grouped to three distinct genotypes. Four samples belonged to ToxoDB Genotype #10 and the other two samples were identified as two new genotypes (http://toxodb.org/toxo/ webcite).

Conclusions

To our knowledge, this is the first report of genetic typing of T. gondii isolates in wildlife on the Qinghai-Tibet Plateau, China. The results show that there is a potential risk for the transmission of this parasite through the wildlife in this region.

【 授权许可】

   
2013 Zhang et al.; licensee BioMed Central Ltd.

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