Genetic variations of ND5 gene of mtDNA in populations of Anopheles sinensis (Diptera: Culicidae) malaria vector in China

Particle and Fibre Toxicology

Qi-Yong Liu²  Shu-Ran Yang³  Xiao-Bo Liu²  Abdelrafie M Makhawi¹ 

[1] Department of Biotechnology, College of Applied and Industrial Sciences, University of Bahri, Khartoum, Sudan;Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China;China CDC Key Laboratory of Surveillance and Early-Warning on Infectious Disease, Chinese Center for Disease Control and Prevention, Beijing 102206, China

关键词: ND5 gene;    mtDNA;    Population genetics structure;    Anopheles sinensis;   

Others  :  824338
DOI  :  10.1186/1756-3305-6-290

 received in 2013-02-12, accepted in 2013-09-24,  发布年份 2013

【 摘 要 】

Background

Anopheles sinensis is a principal vector for Plasmodium vivax malaria in most parts of China. Understanding of genetic structure and genetic differentiation of the mosquito should contribute to the vector control and malaria elimination in China.

Methods

The present study investigated the genetic structure of An. sinensis populations using a 729 bp fragment of mtDNA ND5 among 10 populations collected from seven provinces in China.

Results

ND5 was polymorphic by single mutations within three groups of An. sinensis that were collected from 10 different geographic populations in China. Out of 140 specimens collected from 10 representative sites, 84 haplotypes and 71 variable positions were determined. The overall level of genetic differentiation of An. sinensis varied from low to moderate across China and with a F_ST range of 0.00065 – 0.341. Genealogy analysis clustered the populations of An. sinensis into three main clusters. Each cluster shared one main haplotype. Pairwise variations within populations were higher (68.68%) than among populations (31.32%) and with high fixation index (F_ST = 0.313). The results of the present study support population growth and expansion in the An. sinensis populations from China. Three clusters of An. sinensis populations were detected in this study with each displaying different proportion patterns over seven Chinese provinces. No correlation between genetic and geographic distance was detected in overall populations of An. sinensis (R² = 0.058; P = 0.301).

Conclusions

The results indicate that the ND5 gene of mtDNA is highly polymorphic in An. sinensis and has moderate genetic variability in the populations of this mosquito in China. Demographic and spatial results support evidence of expansion in An. sinensis populations.

【 授权许可】

   
2013 Makhawi et al.; licensee BioMed Central Ltd.

【 图 表 】

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