The colonization of pyrethroid resistant strain from wild Anopheles sinensis, the major Asian malaria vector

Particle and Fibre Toxicology

Qi Gao²  Jun Cao¹  Sui Xu³  Chao Zhang³  Yuanyuan Cao³  Feng Lu³  Yaobao Liu³  Yaping Gu³  Weiming Wang³  Liang Bai³  Jianxia Tang³  Julin Li³  Huayun Zhou³  Guoding Zhu² 

[1] Public Health Research Center, Jiangnan University, Wuxi, People’s Republic of China;Department of Parasitology, Medical College of Soochow University, Suzhou 215123, People’s Republic of China;Key Laboratory of Parasitic Disease Control and Prevention (Ministry of Health), and Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province, People’s Republic of China

关键词: Insecticide resistance;    Pyrethroid;    Anopheles sinensis;   

Others  :  1148545
DOI  :  10.1186/s13071-014-0582-7

 received in 2014-07-25, accepted in 2014-11-30,  发布年份 2014

【 摘 要 】

Background

Anopheles sinensis is one of the most important malaria vectors in Asian countries. The rapid spread of insecticide resistance has become a major obstacle for insecticide-based strategies for vector control. Therefore, it is necessary to prepare an insecticide-resistant strain of An. sinensis to further understand the insecticide resistance mechanisms in this species to facilitate genetic approaches to targeting the insecticide-resistant population of this important malaria vector.

Methods

An. sinensis mosquitoes were collected from regions where pyrethroid resistance had been reported. The mosquitoes were subjected to continuous pyrethroid selection after species confirmation, and the forced copulation method was used to increase the mating rate. In addition, the knockdown-resistance (kdr) mutation frequencies of each generation of An. sinensis were measured; and the metabolic enzyme activities of cytochrome P450 monoxygenases (P450s) and glutathione S-transferases (GSTs) were detected.

Results

The identification of field-captured An. sinensis was confirmed by both morphological and molecular methods. The population of An. sinensis exhibited stable resistance to pyrethroid after continuous generations of pyrethroid selection in the laboratory with high kdr mutation frequencies; and elevated levels of both P450s and GSTs were significantly found in field selected populations comparing with the laboratory susceptible strain. So far, the colonised strain has reached its eleventh generation and culturing well in the laboratory.

Conclusions

We colonised a pyrethroid-resistant population of An. sinensis in the laboratory, which provides a fundamental model for genetic studies of this important malaria vector.

【 授权许可】

   
2014 Zhu et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

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