MicroRNA-10b promotes migration and invasion through Hoxd10 in human gastric cancer

World Journal of Surgical Oncology

Zhi-Long Yan¹  Zhong-Sheng Zhao³  Zai-Yuan Ye¹  Li Li²  Yuan-Yu Wang¹ 

[1] Departments of Gastrointestinal Surgery and Pathology, Zhejiang Provincial People’s Hospital, Hangzhou 310014, People’s Republic of China;Key Laboratory of Gastroenterology of Zhejiang Province, Hangzhou 310014, Zhejiang, People’s Republic of China;Departments of Pathology, Zhejiang Provincial People’s Hospital, Hangzhou 310014, Zhejiang, People’s Republic of China

关键词: Invasion;    Migration;    Proliferation;    Hoxd10;    miR-10b;    Gastric cancer;   

Others  :  1223961
DOI  :  10.1186/s12957-015-0673-8

 received in 2015-03-05, accepted in 2015-08-05,  发布年份 2015

【 摘 要 】

Background

This study aims to investigate the effect of miR-10b overexpression on cancer cell proliferation, migration, invasion, and Hoxd10 expression.

Methods

The effect of miR-10b on proliferation, migration, and invasion of MKN-28, BGC-823, and SGC-7901 cells and the expression of Hoxd10 protein in SGC-7901 and BGC-823 cells were detected following transfection of miR-10b inhibitor or Negative Control B. Expression of Hoxd10 protein in 436 paraffin-embedded cancer tissues was also investigated.

Results

miR-10b was significantly upregulated in AGS, MKN-28, BGC-823, HCG-27, SGC-7901, and MKN-45 cell lines, miR-10b inhibitor significantly inhibited proliferation and migration of MKN-45, BGC-823 and SGC-7901 cells 48 h after transfection, while Hoxd10 protein in these cells lines had increased 72 h after transfection. Hoxd10 was highly expressed in gastric cancer and correlated with size of tumor, Lauren classification, depth of invasion, lymph node and distant metastasis, Tumor-Node-Metastasis (TNM) stage, and prognosis.

Conclusions

miR-10b promotes migration and invasion through Hoxd10 in human gastric cancer cell lines and may play an important role in tumorigenesis, progression, and prognosis.

【 授权许可】

   
2015 Wang et al.

【 图 表 】

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