MicroRNA-10b promotes migration and invasion through Hoxd10 in human gastric cancer

World Journal of Surgical Oncology

Zhi-Long Yan¹  Zhong-Sheng Zhao³  Zai-Yuan Ye¹  Li Li²  Yuan-Yu Wang¹ 

[1] Departments of Gastrointestinal Surgery and Pathology, Zhejiang Provincial People’s Hospital, Hangzhou 310014, People’s Republic of China;Key Laboratory of Gastroenterology of Zhejiang Province, Hangzhou 310014, Zhejiang, People’s Republic of China;Departments of Pathology, Zhejiang Provincial People’s Hospital, Hangzhou 310014, Zhejiang, People’s Republic of China

关键词: Invasion;    Migration;    Proliferation;    Hoxd10;    miR-10b;    Gastric cancer;   

Others  :  1223961
DOI  :  10.1186/s12957-015-0673-8

 received in 2015-03-05, accepted in 2015-08-05,  发布年份 2015

【 摘 要 】

Background

This study aims to investigate the effect of miR-10b overexpression on cancer cell proliferation, migration, invasion, and Hoxd10 expression.

Methods

The effect of miR-10b on proliferation, migration, and invasion of MKN-28, BGC-823, and SGC-7901 cells and the expression of Hoxd10 protein in SGC-7901 and BGC-823 cells were detected following transfection of miR-10b inhibitor or Negative Control B. Expression of Hoxd10 protein in 436 paraffin-embedded cancer tissues was also investigated.

Results

miR-10b was significantly upregulated in AGS, MKN-28, BGC-823, HCG-27, SGC-7901, and MKN-45 cell lines, miR-10b inhibitor significantly inhibited proliferation and migration of MKN-45, BGC-823 and SGC-7901 cells 48 h after transfection, while Hoxd10 protein in these cells lines had increased 72 h after transfection. Hoxd10 was highly expressed in gastric cancer and correlated with size of tumor, Lauren classification, depth of invasion, lymph node and distant metastasis, Tumor-Node-Metastasis (TNM) stage, and prognosis.

Conclusions

miR-10b promotes migration and invasion through Hoxd10 in human gastric cancer cell lines and may play an important role in tumorigenesis, progression, and prognosis.

【 授权许可】

   
2015 Wang et al.

【 图 表 】

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

Fig. 6.

Fig. 7.

Fig. 8.

Fig. 9.

Fig. 10.

【 参考文献 】

• [1]Hutvágner G, Zamore PD. A microRNA in a multiple-turnover RNAi enzyme complex. Science. 2002; 297(5589):2056-2060.
• [2]Bartels CL, Tsongalis GJ. MicroRNAs: novel biomarkers for human cancer. Clin Chem. 2009; 55(4):623-631.
• [3]Ladeiro Y, Couchy G, Balabaud C, Bioulac-Sage P, Pelletier L, Rebouissou S et al.. MicroRNA profiling in hepatocellular tumors is associated with clinical features and oncogene/tumor suppressor gene mutations. Hepatology. 2008; 47(6):1955-1963.
• [4]Bloomston M, Frankel WL, Petrocca F, Volinia S, Alder H, Hagan JP et al.. MicroRNA expression patterns to differentiate pancreatic adenocarcinoma from normal pancreas and chronic pancreatitis. JAMA. 2007; 297(17):1901-1908.
• [5]Tian Y, Luo A, Cai Y, Su Q, Ding F, Chen H et al.. MicroRNA-10b promotes migration and invasion through KLF4 in human esophageal cancer cell lines. J Biol Chem. 2010; 285(11):7986-7994.
• [6]Ma L, Teruya-Feldstein J, Weinberg RA. Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Nature. 2007; 449(7163):682-688.
• [7]Nakata K, Ohuchida K, Mizumoto K, Kayashima T, Ikenaga N, Sakai H et al.. MicroRNA-10b is overexpressed in pancreatic cancer, promotes its invasiveness, and correlates with a poor prognosis. Surgery. 2011; 150(5):916-922.
• [8]Li QJ, Zhou L, Yang F, Wang GX, Zheng H, Wang DS et al.. MicroRNA-10b promotes migration and invasion through CADM1 in human hepatocellular carcinoma cells. Tumour Biol. 2012; 33(5):1455-1465.
• [9]Liu Z, Zhu J, Cao H, Ren H, Fang X. miR-10b promotes cell invasion through RhoC-AKT signaling pathway by targeting HOXD10 in gastric cancer. Int J Oncol. 2012; 40(5):1553-1560.
• [10]Zhao ZS, Wang YY, Chu YQ, Ye ZY, Tao HQ. SPARC is associated with gastric cancer progression and poor survival of patients. Clin Cancer Res. 2010; 16(1):260-268.
• [11]Gabriely G, Teplyuk NM, Krichevsky AM. Context effect: microRNA-10b in cancer cell proliferation, spread and death. Autophagy. 2011; 7(11):1384-1386.
• [12]Liu Y, Zhao J, Zhang PY, Zhang Y, Sun SY, Yu SY et al.. MicroRNA-10b targets E-cadherin and modulates breast cancer metastasis. Med Sci Monit. 2012; 18(8):BR299-BR308.
• [13]Ibrahim SA, Yip GW, Stock C, Pan JW, Neubauer C, Poeter M et al.. Targeting of syndecan-1 by microRNA miR-10b promotes breast cancer cell motility and invasiveness via a Rho-GTPase- and E-cadherin-dependent mechanism. Int J Cancer. 2012; 131(6):E884-E896.
• [14]Sun L, Yan W, Wang Y, Sun G, Luo H, Zhang J et al.. MicroRNA-10b induces glioma cell invasion by modulating MMP-14 and uPAR expression via HOXD10. Brain Res. 2011; 1389:9-18.
• [15]Kim K, Lee HC, Park JL, Kim M, Kim SY, Noh SM et al.. Epigenetic regulation of microRNA-10b and targeting of oncogenic MAPRE1 in gastric cancer. Epigenetics. 2011; 6:740-751.
• [16]Wang YY, Ye ZY, Zhao ZS, Li L, Wang YX, Tao HQ et al.. Clinicopathologic significance of miR-10b expression in gastric carcinoma. Hum Pathol. 2013; 44(7):1278-1285.
• [17]Li Z, Lei H, Luo M, Wang Y, Dong L, Ma Y et al.. DNA methylation downregulated mir-10b acts as a tumor suppressor in gastric cancer. Gastric Cancer. 2015; 18(1):43-54.